Hair & skin derived progenitor cells: In search of a candidate cell for regenerative medicine
نویسندگان
چکیده
BACKGROUND & OBJECTIVES Skin is an established tissue source for cell based therapy. The hair follicle has been introduced later as a tissue source for cell based therapy. The ease of tissue harvest and multipotent nature of the resident stem cells in skin and hair follicle has promoted basic and clinical research in this area. This study was conducted to evaluate skin stem cells (SSCs) and hair follicle stem cells (HFSCs) as candidate cells appropriate for neuronal and melanocyte lineage differentiation. METHODS In this study, SSCs and hair follicle stem cells (HFSCs) were expanded in vitro by explant culture method and were compared in terms of proliferative potential and stemness; differentiation potential into melanocytes and neuronal lineage. RESULTS SSCs were found to be more proliferative in comparison to HFSCs, however, telomerase activity was more in HFSCs in comparison to SSCs. Capacity to differentiate into two lineages of ectoderm origin (neuronal and melanocyte) was found to be different. HFSCs cells showed more propensities towards melanocyte lineage, whereas SSCs were more inclined towards neuronal lineage. INTERPRETATION & CONCLUSIONS The study showed that SSCs had differential advantage over the HFSCs for neuronal cell differentiation, whereas, the HFSCs were better source for melanocytic differentiation.
منابع مشابه
Review Paper: Application of Hair Follicle Bulge Stem Cells in Wound Healing
Despite the significant advances in regenerative medicine, wound healing has remained a challenging clinical problem. Skin is the largest human organ with many vital functions; therefore, any damage to its normal structure should be treated as soon as possible. Easy access to skin stem cells has created a lot of excitement in therapeutic applications. “Cell therapy” is considered a novel method...
متن کاملA New Two Step Induction Protocol for Neural Differentiation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells
Background: In this study, we examined a new two step induction protocol for improving the differentiation of human umbilical cord blood-derived mesenchymal stem cells into neural progenitor cells. Materials and Methods: Human umbilical cord blood-derived mesenchymal stem cells were first cultured in Dulbecco’s modified eagle medium supplemented with 10% fetal bovine serum in a humidified incu...
متن کاملSpermatogonia stem cells: A new pluripotent source for repairment in regenerative medicine
Recently new reports have proved the pluripotency of spermatogonial stem cells (SSCs) derived from male gonad. This pluripotent stem cells resembled Embryonic stem cells recognized as Embryonic Stem like cells (ES like cells). ES like cells forms sharp edge colonies that are immunopositive to pluripotency markers and have differentiation capacity to Ectodermal, Mesodermal and Endodermal layers....
متن کاملAdipose-Derived Stem Cells (ADSC) and Aesthetic Medicine: A Mini Review
In cell therapy and regenerative medicine, a reliable source of stem cells together with cytokine growth factors and biomaterial scaffolds seem necessary. As adipose tissue is easy accessible and is abundant source of adult stem cells and can differentiate along multiple lineages, it can be considered as a good candidate in aesthetic medicine. The clinical application of adipose-derived stem ce...
متن کاملRegenerative Medicine: Highlight on the Significance of Therapeutics with Novel Strategies
Background: There is a worldwide effort to present novel approaches for the development of tolerance-induction treatments in regenerative medicine, after years of investigation in clinical transplantation. Particularly, novel approaches are based on controlling the immune response, including the application of biomaterials or imitation of antigen-specific peripheral tolerance in either solid-or...
متن کامل